Fig. 4
From: A simple and rapid preparation of smooth muscle myosin 2 for the electron microscopic analysis
Step-wise SDS-PAGE and densitometric analysis of smooth muscle myosin-II purification. The proteins were electrophoresed on a 4-20 % gradient gel stained by Coomasie brilliant blue. (S) and (P) demonstrate supernatant and pellet, respectively collected during the washing a, extraction and co-sedimentation with or without phalloidin b steps of the purification after centrifugation. c Comparative densitometric analysis myosin and actin contents between the supernatants of the original actomyosin extract and with (PHD), or without (CONT) phalloidin treatment. d SDS-PAGE profile of eluted fractions from myosin purification using short size exclusion chromatography. S indicates the phalloidin treated supernatant; lanes 1-13 represent eluted fractions. Note that the bands representing Myosin Heavy Chains (HC), Regulatory Light chains (RLC) and Essential Light chains (ELC) and Actin (Ac) have been indicated by arrowheads respectively. The pre-stained protein markers were represented as (M). Note (EXT) designates actomyosin original extract