Fig. 3

TEM analysis of myosin 10S molecules. a Myosin obtained from the supernatant post phalloidin treatment was diluted with ATP, MgCl₂ low salt buffer and cross-linked with 0.1% glutaraldehyde to a final concentration of 100 nM. Black arrows show the head region of the folded myosin molecule. Scale bar, 100 nm. b Alignment and classification of the particles obtained from micrographs was done with 2500 compact particles using SPIDER. The head aligned images of the class averages show the general variance in the orientation of the head and tail. Scale bar, 20 nm