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Applied Microscopy
Fig. 5 | Applied Microscopy

Fig. 5

From: Standardizing HER2 immunohistochemistry assessment: calibration of color and intensity variation in whole slide imaging caused by staining and scanning

Fig. 5

Workflow of HER2 assessment using software and relation between the software and the described method. After color unmixing, cell detection is performed. Average DAB color intensity of each cell membrane is measured and immunoscore is assigned for each cell by comparing of the average DAB color intensity and the thresholds. HER2 status is determined by the percentage of tumor cells in each immunoscore

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