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Applied Microscopy
Fig. 3 | Applied Microscopy

Fig. 3

From: Standardizing HER2 immunohistochemistry assessment: calibration of color and intensity variation in whole slide imaging caused by staining and scanning

Fig. 3

Overview of proposed color and intensity standardization protocol composed of scanner and staining calibration. First, color calibration of the WSI scanner with a color chart slide is performed, then color intensity calibration for staining variation is performed using the IHC calibrator slide. DAB color intensities are obtained from IHC calibrator images (step1–2) and used to calibrate color intensity of tissue images. Method1 is adjusting the thresholds for classifying DAB membranous intensities; Method2 is correcting the color intensity of the image

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