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Applied Microscopy
Fig. 14 | Applied Microscopy

Fig. 14

From: Standardizing HER2 immunohistochemistry assessment: calibration of color and intensity variation in whole slide imaging caused by staining and scanning

Fig. 14

Differences in cell detection results by software in 3 + case. a Linear RGB image; b – d gamma corrected images, uncalibrated, calibrated with IHC-calibrator slide (method:0–2), and calibrated with color chart slide and IHC-calibrator slide (method:1–2); e Comparison of number of cells detected by the software for each input image. Blue: cells with nuclei detected, and orange: cells without nuclei detected

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