Fig. 2
From: Changes of lysosome by L-serine in rotenone-treated hippocampal neurons
L-serine attenuates mitochondrial ROS induced by rotenone. Mitochondria superoxide was detected with 5 μM of MitoSOX with confocal microscopy in HT22 cells. HT22 cells were treated with 10 mM of L-serine for 24 h, 1 μM of rotenone for 19 h and pre-treatment10 mM L-serine for 5 h plus 1 μM of rotenone for 19 h, respectively. A Confocal fluorescence images were taken at 20 min after the addition of MitoSOX. The ROS level was significantly increased by rotenone treatment, But decreased by L-serine treatment. The under image panel is enlarged from the upper yellow box. B Relative quantification of MitoSOX red fluorescence measured by Image J software from 30 cells, respectively. Scale Bar = 20 μm. One-way ANOVA, ****p < 0.0001