Fig. 7

Correlative microscopy. A Schematic drawing of a correlative electron and light microscopy workflow with SEM observation and X-ray microanalysis. B Left: H&E-stained esophageal mucosa containing brown pigment deposition. Bar = 20 μm. Right: Magnified view of a square in the Left. Bar = 10 μm. C SEM observation and X-ray microanalysis. Left: Backscattered electron image. Middle: Lanthanum (La) map. Right: Phosphorous (P) map. Bars = 25 μm. D Weight (Wt) percent of La and P at the bright area (B) and the other dimmer area (D) of the Left panel of (C). From Shinmura et al. 2019 with permission from the publisher