Table 1 Nucleotide sequence of used oligonucleotide primer
Mutation | Sequence (5′➔3′) | Mutant amino acid |
|---|---|---|
K219A(M1) | aF-GAAATTGCCAACGCTCCGAACGGCGAT(AAA ➔ GAA) R-AATCGCCGAGCGGTTCGTTGGCAATTT | Lys ➔ Ala |
K196A(M2) | F-AAAAACTCTCCGGCTAATATTGTGATT (AAA ➔ GAA) R-ATCACAATATTAGCCGGAGAGTTTTTG | Lys ➔ Ala |
K54A(M3) | F-GTCAATCAAAACGGAGCTGCGGTTCAGC (AAA ➔ GAA) R-AGCTGAACCGCAGCTCCGTTTTGATTGA | Lys ➔ Ala |
K157T(M4) | F-TCAAGACTTTATGGCACGACGCCAAACG (AAG ➔ ACG) R-ACGTTTGGCGTCGTGCCATAAAGTCTTG | Lys ➔ Thr |
K75A(M5) | F-CCGTCGAAGCGGCAGCTGAACTCGGAATCT (AAA ➔ GAA) R-GATTCCGAGTTCAGCTGCCGCTTCGACGGC | Lys ➔ Ala |
K119A(M6) | F-ACCAAAACAAAGCGGCTGCAAAAGAATTTTT (AAA ➔ GAA) R-AAAAATTCTTTTGCAGCCGCTTTGTTTTGGT | Lys ➔ Ala |
K143A(M7) | F-CGATTATGTCAACGCTGACTCGTTAAAAT (AAA ➔ GAA) R-TTTTAACGAGGCAGCGTTGACATAATCGC | Lys ➔ Ala |