Fig. 3

Morphological studies of VACV in the era of cryogenic 3DEM. a A cryo-electron micrograph of vitrified VACV mature virion showing surface domain (labeled S) and two membrane boundaries (arrows). b 3D deep-etch microscopy images showing external and internal morphologies of mature VACV virion (top row) and characteristic honeycomb-like scaffold of immature virion (bottom row). c Cryo-electron tomogram of mature VACV virion where top half is shown by surface rendering and bottom half by tomographic section. d Surface representation of cryo-electron tomogram of immature VACV virion. Scale bars are 0.1 μm. The images are reprinted with permission, from a Dubochet et al. Journal of Virology, 1994, doi: https://doi.org/10.1128/jvi.68.3.1935-1941.1994, b Heuser, The Journal of Cell Biology, 2005, doi: https://doi.org/10.1083/jcb.200412169 and Szajner et al., The Journal of Cell Biology, 2005, doi: https://doi.org/10.1083/jcb.200504026, c Cyrklaff et al. PLoS One, 2007, doi: https://doi.org/10.1371/journal.pone.0000420, d Chlanda et al. Cell Host & Microbe, 2009, doi: https://doi.org/10.1016/j.chom.2009.05.021