Fig. 5

Composite intravital microscopy image sequence of a rolling leukocyte (red and green channel). Image sequence showing a sequence of still images (A-D) taken from a video sequence (see: Supplemental Video 1), revealing a rolling leukocyte with several adherent microbeads, emitting fluorescence at 600 nm (red) and 560 nm (green). With an image exposure of 40 ms, the free-flowing fluorescent microbead’s velocity ranged from 12 to 36 μm/s. Microbeads: excitation 488 nm / emission 645 nm and excitation 540 nm / emission 560 nm. Illumination wavelength range: 400–550 nm. Filters: band-pass filter (565/24 nm) and long-pass filter (600 nm). Exposure: 40 ms; frame rate: 16.7 fps. Please note that since all images are captured on monochrome cameras in the MSIVM system, no color images can be generated directly. We used image processing to add color for visualization purposes only. White dotted lines indicate the approximate position of the vascular wall