Fig. 2

Fluorescent microbeads on a glass slide. Images (20X objective; 1000 × 1000 pixels; 93 μm × 93 μm) of microbeads (diameter: 1 μm) on a glass slide, observed with optical channels of the MSIVM system. Images of microbeads with emission at 645 nm are shown for the bight-field channel (400-550 nm, A), the > 600 nm fluorescence channel (B), the 570/25 nm fluorescence channel (C), and the composite channel (D). Images of microbeads with emission at 560 nm are shown for the bright-field channel (400-550 nm, E), the > 600 nm fluorescence channel (F), the 570/25 nm fluorescence channel (G), and the composite channel (H). Simultaneous vizualization of a mixture of two types of fluorescent microbeads (emission at 560 nm and 645 nm) on a glass slide shown for the bright-field channel (I), the > 600 nm fluorescence channel (J), the 570/25 nm fluorescencechannel (K), and the composite channel (L). Microbeads: excitation 488 nm/emission 645 nm and excitation 540 nm/emission 560 nm. Illumination wavelength range: 400–550 nm. Filters: band-pass filter (570/25 nm) and long-pass filter (600 nm). Please note that since all images are captured on monochrome cameras in the MSIVM system, no color images can be generated directly. We used image processing to add color for visualization purposes only