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Table 1 Two methanol fixation protocols for scanning electron microscopy of plant specimens

From: Methanol fixation for scanning electron microscopy of plants

Methanol fixationa

Methanol fixation – ethanol dehydrationb

Immersion in 100% methanol for 30 s

Fixation in 100% methanol for 10 min

Dehydration in 100% ethanol for 30 min

(two times)

Critical point drying

Critical point drying

  1. afrom Neinhuis and Edelmann 1996
  2. bfrom Talbot and White 2013a